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Sample GSM900105 Query DataSets for GSM900105
Status Public on Sep 01, 2012
Title 1_nebulin_+/+ [mRNA]
Sample type RNA
 
Source name mouse WT1 wild type
Organism Mus musculus
Characteristics genotype/variation: nebulin +/+
tissue: quadriceps
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse WT1 with both nebulin alleles
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Mar 23, 2012
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (2)
GSE36743 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [mRNA]
GSE36745 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.589437
AFFX-BioB-M_at 8.811074
AFFX-BioB-3_at 8.616508
AFFX-BioC-5_at 9.44887
AFFX-BioC-3_at 9.762808
AFFX-BioDn-5_at 10.99681
AFFX-BioDn-3_at 11.82933
AFFX-CreX-5_at 13.00824
AFFX-CreX-3_at 12.95728
AFFX-DapX-5_at 9.003876
AFFX-DapX-M_at 10.10284
AFFX-DapX-3_at 10.29073
AFFX-LysX-5_at 6.778169
AFFX-LysX-M_at 7.177035
AFFX-LysX-3_at 7.592851
AFFX-PheX-5_at 7.326707
AFFX-PheX-M_at 7.502143
AFFX-PheX-3_at 7.784174
AFFX-ThrX-5_at 7.632409
AFFX-ThrX-M_at 7.815924

Total number of rows: 16556

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM900105_Witt_150311_WT_1_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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