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Sample GSM900106 Query DataSets for GSM900106
Status Public on Sep 01, 2012
Title 2_nebulin_+/+ [mRNA]
Sample type RNA
 
Source name mouse WT2 wild type
Organism Mus musculus
Characteristics genotype/variation: nebulin +/+
tissue: quadriceps
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse WT2 with both nebulin alleles
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Mar 23, 2012
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (2)
GSE36743 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [mRNA]
GSE36745 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.538232
AFFX-BioB-M_at 8.875503
AFFX-BioB-3_at 8.616264
AFFX-BioC-5_at 9.503373
AFFX-BioC-3_at 9.83229
AFFX-BioDn-5_at 11.05133
AFFX-BioDn-3_at 11.83299
AFFX-CreX-5_at 13.04453
AFFX-CreX-3_at 12.96804
AFFX-DapX-5_at 8.902549
AFFX-DapX-M_at 10.02454
AFFX-DapX-3_at 10.19251
AFFX-LysX-5_at 6.804198
AFFX-LysX-M_at 7.124794
AFFX-LysX-3_at 7.576484
AFFX-PheX-5_at 7.343231
AFFX-PheX-M_at 7.361502
AFFX-PheX-3_at 7.776127
AFFX-ThrX-5_at 7.670021
AFFX-ThrX-M_at 7.792876

Total number of rows: 16556

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM900106_Witt_150311_WT_2_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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