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Sample GSM900107 Query DataSets for GSM900107
Status Public on Sep 01, 2012
Title 3_nebulin_+/+ [mRNA]
Sample type RNA
Source name mouse WT3 wild type
Organism Mus musculus
Characteristics genotype/variation: nebulin +/+
tissue: quadriceps
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse WT3 with both nebulin alleles
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
Submission date Mar 23, 2012
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platform ID GPL14661
Series (2)
GSE36743 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [mRNA]
GSE36745 In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy

Data table header descriptions
VALUE RMA signal intensity

Data table
AFFX-BioB-5_at 8.481564
AFFX-BioB-M_at 8.896449
AFFX-BioB-3_at 8.627803
AFFX-BioC-5_at 9.459018
AFFX-BioC-3_at 9.791708
AFFX-BioDn-5_at 11.01887
AFFX-BioDn-3_at 11.81313
AFFX-CreX-5_at 13.02052
AFFX-CreX-3_at 12.93388
AFFX-DapX-5_at 8.859757
AFFX-DapX-M_at 9.893645
AFFX-DapX-3_at 10.12494
AFFX-LysX-5_at 6.812025
AFFX-LysX-M_at 7.158132
AFFX-LysX-3_at 7.508489
AFFX-PheX-5_at 7.204789
AFFX-PheX-M_at 7.328345
AFFX-PheX-3_at 7.649918
AFFX-ThrX-5_at 7.633948
AFFX-ThrX-M_at 7.677962

Total number of rows: 16556

Table truncated, full table size 295 Kbytes.

Supplementary file Size Download File type/resource
GSM900107_Witt_150311_WT_3_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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