|
| Status |
Public on May 10, 2012 |
| Title |
VE-Cadherin+/CD45+ cells from Tie-2 Etv2 Embryos Yolk sac (YS) rep1 |
| Sample type |
RNA |
| |
|
| Source name |
YS from Tie-2-Etv2(Etv2 Tg turned ON by Tie-2 Cre Tg) embryo
|
| Organism |
Mus musculus |
| Characteristics |
cell type: VE-Cadherin+/CD45+
strain: mixed (B6/CBA and others)
genotype/variation: Tie-2-Etv2
tissue: yolk sac
|
| Biomaterial provider |
Knock-in and Tg mice crossed at CDB RIKEN
|
| Treatment protocol |
FACS sort only, no treatment afterwards
|
| Growth protocol |
FACS sort only, no treatment afterwards
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We used Qiagen RNA microelute Kit.
|
| Label |
Cy3
|
| Label protocol |
We used Agilent Low Input Quick Amp Labeling Kit, and followed manufacturer's protocol.
http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf
|
| |
|
| Hybridization protocol |
We used Agilent Hybridization Oven, and followed manufacturer's protocol.
http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf
|
| Scan protocol |
We used Agilent Microarray Scanner, and followed manufacturer's protocol.
http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf
|
| Data processing |
We used Agilent Feature Extraction 10.7 for quantification, and GeneSpring GX11.5.1 for data analysis.
1. Data Transformation: Set measurements less than 1 to 1
2. Per Chip: Normalize to 75 percentile
3. Per Gene: Normalize to median
|
| |
|
| Submission date |
Mar 26, 2012 |
| Last update date |
May 10, 2012 |
| Contact name |
Hiroshi Kataoka |
| Organization name |
Riken Center for Developmental Biology
|
| Department |
Stem Cell Biology
|
| Street address |
2-2-3 Minatojima-minamimachi, Chuo-ku
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE36788 |
Gene expression profiling in Tie-2-Etv2 YS hematopoietic precursors |
|
