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Sample GSM901271 Query DataSets for GSM901271
Status Public on Jun 21, 2012
Title DKO4
Sample type RNA
 
Source name DKO_embryonic heart
Organism Mus musculus
Characteristics strain background: C57/BL6;129Ola mixed
genotype/variation: MesP1-cre:RBPJlox/lox//Axin2-/- (DKO)
age: 22-24somites
developmental stage: E9.25
tissue: embryonic heart
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplif Kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 5388876038_d
Data processing The data have been normalized using the BeadStudio Gene Expression Module v3.3.7, and quantile normalization without background correction. Not or low expressed transcripts have been removed by a minimum Illumina detection p-value < 0.05. The data filtering resulted in 14,788 of 25,697 probes.
 
Submission date Mar 26, 2012
Last update date Jun 21, 2012
Contact name Alexandra Klaus
E-mail(s) aklaus@mdc-berlin.de
Phone +49 30 9406 3791
Fax +49 30 9406 2656
Organization name MDC (Max Delbrück Center)
Department Signal Transduction, Invasion and Metastasis of Epithelial Cells
Lab Prof.Walter Birchmeier
Street address Robert-Roessle-Str.10
City Berlin
State/province Berlin
ZIP/Postal code 13125
Country Germany
 
Platform ID GPL6885
Series (1)
GSE36804 Expression analysis of genes regulated by RBPJ (Notch) and Axin2 (Wnt) mediated signaling in mouse cardiogenesis at E9.25

Data table header descriptions
ID_REF
VALUE quantile normalized (without background correction)

Data table
ID_REF VALUE
ILMN_1212607 175.3241
ILMN_1212612 181.7691
ILMN_1212632 265.524
ILMN_1212636 11245.96
ILMN_1212637 3093.581
ILMN_1212645 191.0863
ILMN_1212648 652.0725
ILMN_1212653 308.5738
ILMN_1212672 915.402
ILMN_1212682 207.8048
ILMN_1212692 750.3156
ILMN_1212693 377.7562
ILMN_1212702 40468.4
ILMN_1212703 293.4519
ILMN_1212717 506.6203
ILMN_1212720 801.6907
ILMN_1212759 626.5435
ILMN_1212791 261.9999
ILMN_1212794 382.3854
ILMN_1212823 276.4088

Total number of rows: 14788

Table truncated, full table size 316 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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