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Sample GSM901274 Query DataSets for GSM901274
Status Public on Jun 21, 2012
Title DKOhet1
Sample type RNA
 
Source name DKOhet_embryonic heart
Organism Mus musculus
Characteristics strain background: C57/BL6;129Ola mixed
genotype/variation: MesP1-cre:RBPJlox/lox//Axin2+/- (DKOhet)
age: 22-24somites
developmental stage: E9.25
tissue: embryonic heart
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplif Kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 5388876038_e
Data processing The data have been normalized using the BeadStudio Gene Expression Module v3.3.7, and quantile normalization without background correction. Not or low expressed transcripts have been removed by a minimum Illumina detection p-value < 0.05. The data filtering resulted in 14,788 of 25,697 probes.
 
Submission date Mar 26, 2012
Last update date Jun 21, 2012
Contact name Alexandra Klaus
E-mail(s) aklaus@mdc-berlin.de
Phone +49 30 9406 3791
Fax +49 30 9406 2656
Organization name MDC (Max Delbrück Center)
Department Signal Transduction, Invasion and Metastasis of Epithelial Cells
Lab Prof.Walter Birchmeier
Street address Robert-Roessle-Str.10
City Berlin
State/province Berlin
ZIP/Postal code 13125
Country Germany
 
Platform ID GPL6885
Series (1)
GSE36804 Expression analysis of genes regulated by RBPJ (Notch) and Axin2 (Wnt) mediated signaling in mouse cardiogenesis at E9.25

Data table header descriptions
ID_REF
VALUE quantile normalized (without background correction)

Data table
ID_REF VALUE
ILMN_1212607 158.7521
ILMN_1212612 157.9221
ILMN_1212632 257.0759
ILMN_1212636 9999.385
ILMN_1212637 3923.557
ILMN_1212645 224.0326
ILMN_1212648 538.7797
ILMN_1212653 371.4495
ILMN_1212672 856.1058
ILMN_1212682 187.4986
ILMN_1212692 710.6476
ILMN_1212693 420.6224
ILMN_1212702 62646.92
ILMN_1212703 287.278
ILMN_1212717 524.2438
ILMN_1212720 1021.019
ILMN_1212759 609.7898
ILMN_1212791 223.1742
ILMN_1212794 281.198
ILMN_1212823 259.9219

Total number of rows: 14788

Table truncated, full table size 316 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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