|
| Status |
Public on Jun 21, 2012 |
| Title |
DKOhet2 |
| Sample type |
RNA |
| |
|
| Source name |
DKOhet_embryonic heart
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57/BL6;129Ola mixed
genotype/variation: MesP1-cre:RBPJlox/lox//Axin2+/- (DKOhet)
age: 22-24somites
developmental stage: E9.25
tissue: embryonic heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplif Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5388876038_h
|
| Data processing |
The data have been normalized using the BeadStudio Gene Expression Module v3.3.7, and quantile normalization without background correction. Not or low expressed transcripts have been removed by a minimum Illumina detection p-value < 0.05. The data filtering resulted in 14,788 of 25,697 probes.
|
| |
|
| Submission date |
Mar 26, 2012 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Alexandra Klaus |
| E-mail(s) |
aklaus@mdc-berlin.de
|
| Phone |
+49 30 9406 3791
|
| Fax |
+49 30 9406 2656
|
| Organization name |
MDC (Max Delbrück Center)
|
| Department |
Signal Transduction, Invasion and Metastasis of Epithelial Cells
|
| Lab |
Prof.Walter Birchmeier
|
| Street address |
Robert-Roessle-Str.10
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE36804 |
Expression analysis of genes regulated by RBPJ (Notch) and Axin2 (Wnt) mediated signaling in mouse cardiogenesis at E9.25 |
|
