|
| Status |
Public on May 01, 2012 |
| Title |
Methyl sufficient diet mouse colon 1 sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
CONTROL
|
| Organism |
Mus musculus |
| Characteristics |
strain: ApcMin
tissue: colon
|
| Treatment protocol |
ApcMin mice were fed methyl deficient diets (MDD) or methyl sufficient diet MSD (CONTROL) for 11 weeks and normal colons were harvested for expression profiling, duplicate samples were taken from each animal
2 control animals and 4 methyl deficient animals were used for expression profiling
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Colon RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Methyl sufficient diet mouse colon 1 sample 2
|
| Data processing |
The data were normalised using 75th percentile shift in Genespring GX
|
| |
|
| Submission date |
Apr 03, 2012 |
| Last update date |
May 01, 2012 |
| Contact name |
Petr Protiva |
| E-mail(s) |
petr.protiva@yale.edu
|
| Phone |
230-932-5711
|
| Organization name |
Yale University and VA CT Healthcare System
|
| Street address |
950 Campbell Avenue
|
| City |
West Haven |
| ZIP/Postal code |
06516 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE37010 |
Effect of dietary methyl donor depletion on gene expression in normal colons of ApcMin mice |
|
