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Sample GSM911813 Query DataSets for GSM911813
Status Public on Jul 12, 2012
Title 732liver
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics tissue: Liver
strain: C57BL/6
age: 48 weeks
treatment: RAD001
Treatment protocol At ~44 weeks of age, the animals (n=9-10) were gavaged twice daily for 28 days with the specified doses of RAD001 and/or BEZ235. The mice were starved for three hours prior to sacrifice and received their last dose of RAD001 at the time starvation or BEZ235 1 hour before sacrifice.
Growth protocol C57Bl/6 mice were obtained from Jackson laboratories. At two weeks of age, male mice were injected intraperitoneally with 50 mg/kg BW of diethylnitrosamine (DEN) (Sigma).
Extracted molecule total RNA
Extraction protocol Total RNA from all the samples was purified using the Qiagen RNeasy purification kit following the manufacturer’s protocol.
Label biotin
Label protocol The integrity of the RNA was measured using a Bioanalyser and RIN values > 7-8 were considered adequate to proceed further with total RNA transcription into cRNA using the Illumina Total Prep RNA amplification kit (Ambion) following the manufacturer’s protocol.
 
Hybridization protocol Biotinylated cRNA was hybridized to Illumina MouseRef8v2 bead chips.
Scan protocol Gene expression matrices were scanned using Illumina iSCAN Control Software.
Description 10 mg/Kg BW
Data processing Data was quantile normalized with background subtraction using Illumina BeadStudio.
 
Submission date Apr 09, 2012
Last update date Jul 12, 2012
Contact name Manway Liu
E-mail(s) manway.liu@novartis.com
Organization name Novartis Institutes for Biomedical Research
Street address 250 Massachusetts Avenue
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL6885
Series (1)
GSE37129 mTOR inhibitors synergistically induce regression, reversal of gene expression and autophagy in hepatocellular carcinoma

Data table header descriptions
ID_REF
VALUE Quantile normalization with background subtraction.
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 6.707185 0.1679198
ILMN_1212612 47.06477 0
ILMN_1212619 0.3717028 0.4172932
ILMN_1212628 10.29996 0.09774436
ILMN_1212632 39.46974 0
ILMN_1212636 1430.965 0
ILMN_1212637 1082.525 0
ILMN_1212645 8063.617 0
ILMN_1212648 95.06612 0
ILMN_1212653 19.94462 0.02756892
ILMN_1212672 190.9076 0
ILMN_1212682 14.9478 0.05012531
ILMN_1212683 0.1416259 0.4273183
ILMN_1212685 0.1212648 0.4285714
ILMN_1212692 8.203241 0.1378446
ILMN_1212693 41.39022 0
ILMN_1212695 17.83833 0.03383459
ILMN_1212698 -2.286785 0.5689223
ILMN_1212702 16799.23 0
ILMN_1212703 151.1629 0

Total number of rows: 25697

Table truncated, full table size 747 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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