|
| Status |
Public on Aug 18, 2012 |
| Title |
NFATc1-deficient osteoclasts rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse osteoclast culture, day 3 MCSF/RANKL
|
| Organism |
Mus musculus |
| Characteristics |
cell type: bone marrow macrophage derived osteoclasts
strain: C57BL/6
genotype: NFATc1 fl/fl MxCre+
|
| Treatment protocol |
Osteoclat precursor cultures were cultured in the presence of MCSF and RANKL for 3 days to generate early osteoclasts.
|
| Growth protocol |
Osteoclast precursors were purified from whole bone marrow of n=3 mice (replicate 1) or n=4 mice (replicate 2) by flow cytometric sorting for CD11b- CD3e- B220- ckit+cfms+ cell s and plated at 20,000/24well plate in alpha-meM with mCSF and RANKL.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression data in osteoclast cultures
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 12, 2012 |
| Last update date |
Aug 19, 2012 |
| Contact name |
Julia F. Charles |
| E-mail(s) |
jfcharles@partners.org
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Division of Rheumatology, Immunology and Allergy
|
| Lab |
Antonios O. Aliprantis Lab
|
| Street address |
75 Francis Street, Smith Building 6th floor
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE37219 |
Comparison expression data from wild-type and NFATc1-deficient osteoclasts |
|
