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Sample GSM914182 Query DataSets for GSM914182
Status Public on Apr 13, 2012
Title 82743-lo
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics background strain: C57BL/6
tissue: tumor
genotype/variation: MYCN AND mutated ALK
Treatment protocol none
Growth protocol Mice transgenic for neural crest specific expression of MYCN, mutated ALK or both were monitored until tumor was palpable and sacrificed afterwards.
Extracted molecule total RNA
Extraction protocol RNA was extracted from tumors or control tissue using the QIAGEN Rneasy mini kit.
Label biotin
Label protocol Biotin labelling was performed according the manufacturer's recommendations (Affymetrix).
 
Hybridization protocol Hybridisation to mouse 430_2 arrays was performed according to the manufacturer's recommendations (Affymetrix).
Scan protocol Scanning of chips was performed using a GeneArray scanner (Agilent).
Description tumor driven by MYCN AND mutated ALK
Data processing Signal intensities were RMA normalized using PARTEK Genomics Suite, with the .CEL files as input.
 
Submission date Apr 13, 2012
Last update date Apr 13, 2012
Contact name Alexander Schramm
E-mail(s) alexander.schramm@uk-essen.de
Organization name University Hospital Essen
Department Childrens Hospital
Lab Hematology/Oncology Lab
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45122
Country Germany
 
Platform ID GPL1261
Series (1)
GSE32386 Expression profiling of murine neuroblastoma in transgenic mice

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity

Data table
ID_REF VALUE
1415670_at 8.89756
1415671_at 11.3303
1415672_at 11.4286
1415673_at 7.69626
1415674_a_at 9.94619
1415675_at 9.08617
1415676_a_at 11.5081
1415677_at 9.1314
1415678_at 10.3357
1415679_at 10.9472
1415680_at 8.97537
1415681_at 9.83229
1415682_at 8.278
1415683_at 10.1304
1415684_at 8.83368
1415685_at 9.28193
1415686_at 10.087
1415687_a_at 11.7631
1415688_at 10.557
1415689_s_at 9.49803

Total number of rows: 45101

Table truncated, full table size 849 Kbytes.




Supplementary file Size Download File type/resource
GSM914182_82743-lo_10_Mouse430_2_.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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