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Sample GSM914183 Query DataSets for GSM914183
Status Public on Apr 13, 2012
Title 77204-10
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics background strain: C57BL/6
tissue: tumor
genotype/variation: MYCN AND mutated ALK
Treatment protocol none
Growth protocol Mice transgenic for neural crest specific expression of MYCN, mutated ALK or both were monitored until tumor was palpable and sacrificed afterwards.
Extracted molecule total RNA
Extraction protocol RNA was extracted from tumors or control tissue using the QIAGEN Rneasy mini kit.
Label biotin
Label protocol Biotin labelling was performed according the manufacturer's recommendations (Affymetrix).
 
Hybridization protocol Hybridisation to mouse 430_2 arrays was performed according to the manufacturer's recommendations (Affymetrix).
Scan protocol Scanning of chips was performed using a GeneArray scanner (Agilent).
Description tumor driven by MYCN AND mutated ALK
Data processing Signal intensities were RMA normalized using PARTEK Genomics Suite, with the .CEL files as input.
 
Submission date Apr 13, 2012
Last update date Apr 13, 2012
Contact name Alexander Schramm
E-mail(s) alexander.schramm@uk-essen.de
Organization name University Hospital Essen
Department Childrens Hospital
Lab Hematology/Oncology Lab
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45122
Country Germany
 
Platform ID GPL1261
Series (1)
GSE32386 Expression profiling of murine neuroblastoma in transgenic mice

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity

Data table
ID_REF VALUE
1415670_at 9.0125
1415671_at 11.5445
1415672_at 11.4124
1415673_at 7.60943
1415674_a_at 9.67276
1415675_at 9.03469
1415676_a_at 11.375
1415677_at 8.49217
1415678_at 10.7794
1415679_at 11.0821
1415680_at 9.03058
1415681_at 9.99459
1415682_at 8.23517
1415683_at 10.2239
1415684_at 8.6729
1415685_at 8.98251
1415686_at 10.0126
1415687_a_at 11.8059
1415688_at 10.771
1415689_s_at 9.25533

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM914183_77204-10_8_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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