|
| Status |
Public on Jan 12, 2006 |
| Title |
UASey_legdisc_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
third instar leg discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Third instar lavae leg discs from UAS-ey/dpp-GAL4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol total RNA
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis was carried out as described in the Expression Analysis Technical Manual (Affymetrix, two-cycle protocol) using 100 ng of total RNA for each sample. The cRNA reactions were carried out using the BioArray High-Yield Transcript Labeling kit (Enzo).
|
| |
|
| Hybridization protocol |
Fifteen micrograms of labeled cRNA was fragmented and sequentially hybridized to the Affymetrix Drosophila Genome Array 2 following the manufacturer’s instructions.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Genome-Wide Identification of Direct Targets of the Drosophila Retinal Determination Protein Eyeless
|
| Data processing |
dChip
|
| |
|
| Submission date |
Jan 10, 2006 |
| Last update date |
Mar 12, 2007 |
| Contact name |
rui chen |
| E-mail(s) |
ruichen@bcm.tmc.edu
|
| Phone |
7137985194
|
| Organization name |
Baylor College of Medicine
|
| Department |
HGSC, Department of Molecular and Human Gentics
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE4008 |
Genome-Wide Identification of Direct Targets of the Drosophila Retinal Determination Protein Eyeless |
|
