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Sample GSM91480 Query DataSets for GSM91480
Status Public on Jan 11, 2006
Title UASey_legdisc_rep3
Sample type RNA
 
Source name third instar leg discs
Organism Drosophila melanogaster
Characteristics Third instar lavae leg discs from UAS-ey/dpp-GAL4
Extracted molecule total RNA
Extraction protocol Trizol total RNA
Label biotin
Label protocol cDNA synthesis was carried out as described in the Expression Analysis Technical Manual (Affymetrix, two-cycle protocol) using 100 ng of total RNA for each sample. The cRNA reactions were carried out using the BioArray High-Yield Transcript Labeling kit (Enzo).
 
Hybridization protocol Fifteen micrograms of labeled cRNA was fragmented and sequentially hybridized to the Affymetrix Drosophila Genome Array 2 following the manufacturer’s instructions.
Scan protocol Standard Affymetrix protocol.
Description Genome-Wide Identification of Direct Targets of the Drosophila Retinal Determination Protein Eyeless
Data processing dChip
 
Submission date Jan 10, 2006
Last update date Mar 12, 2007
Contact name rui chen
E-mail(s) ruichen@bcm.tmc.edu
Phone 7137985194
Organization name Baylor College of Medicine
Department HGSC, Department of Molecular and Human Gentics
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL1322
Series (1)
GSE4008 Genome-Wide Identification of Direct Targets of the Drosophila Retinal Determination Protein Eyeless

Data table header descriptions
ID_REF
VALUE probe set value

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.89613
AFFX-BioB-M_at 8.85462
AFFX-BioB-3_at 8.9018
AFFX-BioC-5_at 9.62563
AFFX-BioC-3_at 9.31533
AFFX-BioDn-5_at 10.4197
AFFX-BioDn-3_at 12.0332
AFFX-CreX-5_at 13.0582
AFFX-CreX-3_at 13.483
AFFX-DapX-5_at 7.40574
AFFX-DapX-M_at 9.6559
AFFX-DapX-3_at 11.0161
AFFX-LysX-5_at 6.91296
AFFX-LysX-M_at 7.8988
AFFX-LysX-3_at 9.13879
AFFX-PheX-5_at 7.12008
AFFX-PheX-M_at 8.07092
AFFX-PheX-3_at 9.02681
AFFX-ThrX-5_at 7.17598
AFFX-ThrX-M_at 7.68837

Total number of rows: 18952

Table truncated, full table size 358 Kbytes.




Supplementary data files not provided

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