|
Status |
Public on Apr 20, 2012 |
Title |
normal control_CTL3 |
Sample type |
RNA |
|
|
Source name |
normal control
|
Organism |
Homo sapiens |
Characteristics |
tissue: colon biopsie
|
Extracted molecule |
total RNA |
Extraction protocol |
Rneasy kit (Qiagen, Hilden, Germany)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix human gut microbiota community GeneChip, then washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
S12 Gene expression data from human colon biopsy
|
Data processing |
Quality check and Robust Multichip Normalization using R/Bioconductor affy package
|
|
|
Submission date |
Apr 16, 2012 |
Last update date |
Apr 20, 2012 |
Contact name |
Yong Huang |
E-mail(s) |
yongh@uchicago.edu
|
Phone |
773-834-0832
|
Organization name |
University of Chicago
|
Department |
Medicine
|
Street address |
900 East 57th Street
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL13158 |
Series (1) |
GSE37283 |
Gene Expression Changes in Non-Dysplastic Mucosa from Patients with Ulcerative Colitis Harboring Remote Neoplastic Lesions |
|