|
| Status |
Public on Apr 20, 2012 |
| Title |
ulcerative colitis with neoplasia_UCD1 |
| Sample type |
RNA |
| |
|
| Source name |
ulcerative colitis with neoplasia
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colon biopsie
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix human gut microbiota community GeneChip, then washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
S8
Gene expression data from human colon biopsy
|
| Data processing |
Quality check and Robust Multichip Normalization using R/Bioconductor affy package
|
| |
|
| Submission date |
Apr 16, 2012 |
| Last update date |
Apr 20, 2012 |
| Contact name |
Yong Huang |
| E-mail(s) |
yongh@uchicago.edu
|
| Phone |
773-834-0832
|
| Organization name |
University of Chicago
|
| Department |
Medicine
|
| Street address |
900 East 57th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE37283 |
Gene Expression Changes in Non-Dysplastic Mucosa from Patients with Ulcerative Colitis Harboring Remote Neoplastic Lesions |
|
