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Sample GSM92220 Query DataSets for GSM92220
Status Public on Mar 31, 2006
Title BN DES-treated replicate 4
Sample type RNA
 
Source name anterior pituitary
Organism Rattus norvegicus
Characteristics BN strain, male, age 21 weeks, treated with the synthetic estrogen diethylstilbestrol (DES) for 12 weeks
Extracted molecule total RNA
Extraction protocol Anterior pituitary gland was harvested at the time of sacrifice, weighed and flash-frozen in liquid nitrogen. Tissue was homogenized in lysis buffer by passage through an 18- and then 22-guage needle. Total RNA was isolated using the Absolutely RNA Kit from Stratagene.
Label biotin
Label protocol Approximately 2 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description BN rats were obtained from Harlan (Indianapolis, IN). At 9 weeks of age, a silastic tubing implant containing 5mg of the synthetic estrogen diethylstilbestrol (DES) was surgically implanted subcutaneously in the interscapular region of male rats. Each animal received an intraperitoneal injection of BrdU (50mg/kg) 4 hours prior to sacrifice. Animals were sacrificed at 21 weeks of age by decapitation and pituitary glands were immediately harvested, weighed and flash-frozen in liquid nitrogen. Tissues were homogenized through a 22-gauge needle and total RNA was extracted using the Absolutely RNA Kit (Stratagene). A total of 4 DES-treated males were used, resulting in three independent biological replicates.
Data processing Affymetrix GeneChip Operating Software version 1.2
 
Submission date Jan 13, 2006
Last update date Nov 27, 2012
Contact name James Shull
E-mail(s) shull@oncology.wisc.edu
Phone 608-265-5003
Organization name University of Wisconsin-Madison
Department McArdle Laboratory for Cancer Research
Lab Shull Laboratory
Street address 1400 University Avenue
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platform ID GPL1355
Series (2)
GSE4028 Effects of diethylstilbestrol (DES) on the anterior pituitary gland of the ACI, Copenhagen and Brown Norway Rat.
GSE4083 Diethylstilbestrol effects on rat anterior pituitary gland

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 653.9 P 0.000754
AFFX-BioB-M_at 911.3 P 0.000044
AFFX-BioB-3_at 555.8 P 0.000044
AFFX-BioC-5_at 2040.2 P 0.000044
AFFX-BioC-3_at 2159.8 P 0.000052
AFFX-BioDn-5_at 3603.3 P 0.00006
AFFX-BioDn-3_at 9103.3 P 0.000052
AFFX-CreX-5_at 20176.6 P 0.000044
AFFX-CreX-3_at 27265.1 P 0.000044
AFFX-DapX-5_at 2144.2 P 0.000044
AFFX-DapX-M_at 4283.4 P 0.00007
AFFX-DapX-3_at 5912.7 P 0.000044
AFFX-LysX-5_at 227.5 P 0.00011
AFFX-LysX-M_at 460.3 P 0.000754
AFFX-LysX-3_at 1042.9 P 0.000052
AFFX-PheX-5_at 473.8 P 0.000052
AFFX-PheX-M_at 510.6 P 0.000044
AFFX-PheX-3_at 587.6 P 0.00034
AFFX-ThrX-5_at 538.8 P 0.000147
AFFX-ThrX-M_at 664.1 P 0.000044

Total number of rows: 31099

Table truncated, full table size 831 Kbytes.




Supplementary data files not provided

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