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Sample GSM923107 Query DataSets for GSM923107
Status Public on Apr 27, 2012
Title Day8_Control
Sample type RNA
 
Source name Mouse embryonic stem cell (G6B-2)
Organism Mus musculus
Characteristics tissue: Mouse blastocyst
cell type: Mouse embryonic stem cell (G6B-2)
treatment/growth condition: Expose 0.1% Ethanol for 4days between Day2 and Day6
Treatment protocol Retinoic acid were treated into the culture media at the iniciated stage after forming embryonic body
Growth protocol Embryoid bodies formed from nouse ES cells and differentiated into Neural cells by differentiation-inducing medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with the QIAGEN RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Expose 0.1% Ethanol for 4days between Day2 and Day6
Data processing The data were normalised using quantile normalisation with GeneSpring GX11(Agilent technologies)
 
Submission date Apr 26, 2012
Last update date Apr 27, 2012
Contact name Sone Hideko
E-mail(s) hsone@nies.go.jp
Phone 81298502420
Organization name National Institute for Environmental Studies
Department Center for Environmental Risk Research
Lab Environmental Exposure Research Section
Street address onogawa16-2
City tsukuba
State/province ibaraki
ZIP/Postal code 305-8506
Country Japan
 
Platform ID GPL6481
Series (1)
GSE37602 Identification of stage-specific gene expression signatures in response to retinoic acid during the neural differentiation of mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
10243 0.02305317 0.01122895
10280 0.28497648 0.03992514
10575 0.12089205 0.003119152
20048 0.38857603 0.008109794
20296 -0.06881428 0
20343 -0.050940752 0
20373 -0.70064116 0.2158453
20431 -0.044116974 0
50014 0.7573199 0.3156582
50017 -0.1856153 0.1185278
50019 0.0297091 0
50020 -0.3218465 0.008109794
50022 0.79495454 0.03992514
50025 1.2555351 0.2869619
50026 0.3457327 0.3643169
50035 -0.24268007 0.4840923
50037 -0.08386874 0.00062383
50039 -0.18423629 0
50040 -0.40704417 0.01746725
50041 -0.672354 0.004990642

Total number of rows: 46643

Table truncated, full table size 1327 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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