|
| Status |
Public on Apr 27, 2012 |
| Title |
Day8_RA10-9M |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic stem cell (G6B-2)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Mouse blastocyst
cell type: Mouse embryonic stem cell (G6B-2)
treatment/growth condition: Expose 10-9M Retinoic acid for 4days between Day2 and Day6
|
| Treatment protocol |
Retinoic acid were treated into the culture media at the iniciated stage after forming embryonic body
|
| Growth protocol |
Embryoid bodies formed from nouse ES cells and differentiated into Neural cells by differentiation-inducing medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Expose 10-9M Retinoic acid for 4days between Day2 and Day6
|
| Data processing |
The data were normalised using quantile normalisation with GeneSpring GX11(Agilent technologies)
|
| |
|
| Submission date |
Apr 26, 2012 |
| Last update date |
Apr 27, 2012 |
| Contact name |
Sone Hideko |
| E-mail(s) |
hsone@nies.go.jp
|
| Phone |
81298502420
|
| Organization name |
National Institute for Environmental Studies
|
| Department |
Center for Environmental Risk Research
|
| Lab |
Environmental Exposure Research Section
|
| Street address |
onogawa16-2
|
| City |
tsukuba |
| State/province |
ibaraki |
| ZIP/Postal code |
305-8506 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE37602 |
Identification of stage-specific gene expression signatures in response to retinoic acid during the neural differentiation of mouse embryonic stem cells |
|
