|
Status |
Public on Apr 27, 2012 |
Title |
Day8_RA10-7M |
Sample type |
RNA |
|
|
Source name |
Mouse embryonic stem cell (G6B-2)
|
Organism |
Mus musculus |
Characteristics |
tissue: Mouse blastocyst cell type: Mouse embryonic stem cell (G6B-2) treatment/growth condition: Expose 10-7M Retinoic acid for 4days between Day2 and Day6
|
Treatment protocol |
Retinoic acid were treated into the culture media at the iniciated stage after forming embryonic body
|
Growth protocol |
Embryoid bodies formed from nouse ES cells and differentiated into Neural cells by differentiation-inducing medium.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with the QIAGEN RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Expose 10-7M Retinoic acid for 4days between Day2 and Day6
|
Data processing |
The data were normalised using quantile normalisation with GeneSpring GX11(Agilent technologies)
|
|
|
Submission date |
Apr 26, 2012 |
Last update date |
Apr 27, 2012 |
Contact name |
Sone Hideko |
E-mail(s) |
hsone@nies.go.jp
|
Phone |
81298502420
|
Organization name |
National Institute for Environmental Studies
|
Department |
Center for Environmental Risk Research
|
Lab |
Environmental Exposure Research Section
|
Street address |
onogawa16-2
|
City |
tsukuba |
State/province |
ibaraki |
ZIP/Postal code |
305-8506 |
Country |
Japan |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE37602 |
Identification of stage-specific gene expression signatures in response to retinoic acid during the neural differentiation of mouse embryonic stem cells |
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