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Sample GSM923110 Query DataSets for GSM923110
Status Public on Apr 27, 2012
Title Day8_RA10-7M
Sample type RNA
 
Source name Mouse embryonic stem cell (G6B-2)
Organism Mus musculus
Characteristics tissue: Mouse blastocyst
cell type: Mouse embryonic stem cell (G6B-2)
treatment/growth condition: Expose 10-7M Retinoic acid for 4days between Day2 and Day6
Treatment protocol Retinoic acid were treated into the culture media at the iniciated stage after forming embryonic body
Growth protocol Embryoid bodies formed from nouse ES cells and differentiated into Neural cells by differentiation-inducing medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with the QIAGEN RLT buffer, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Expose 10-7M Retinoic acid for 4days between Day2 and Day6
Data processing The data were normalised using quantile normalisation with GeneSpring GX11(Agilent technologies)
 
Submission date Apr 26, 2012
Last update date Apr 27, 2012
Contact name Sone Hideko
E-mail(s) hsone@nies.go.jp
Phone 81298502420
Organization name National Institute for Environmental Studies
Department Center for Environmental Risk Research
Lab Environmental Exposure Research Section
Street address onogawa16-2
City tsukuba
State/province ibaraki
ZIP/Postal code 305-8506
Country Japan
 
Platform ID GPL6481
Series (1)
GSE37602 Identification of stage-specific gene expression signatures in response to retinoic acid during the neural differentiation of mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
10243 0.0406065 0.01310044
10280 -0.23004055 0.06425452
10575 -0.22439337 0.004366812
20048 0.049539566 0.01310044
20296 -0.11293936 0
20343 -0.4952209 0.00062383
20373 -0.19861746 0.1291329
20431 0.6125703 0
50014 -0.30137157 0.6419214
50017 -0.02768302 0.08109794
50019 -0.24701524 0
50020 0.46310377 0.003119152
50022 -2.953036 0.4148472
50025 -0.11349678 0.5433562
50026 -0.11349678 0.9656894
50035 0.39462233 0.3275109
50037 -0.5647166 0.002495321
50039 -0.12567925 0
50040 0.19105077 0.007485964
50041 0.08554673 0.00062383

Total number of rows: 46643

Table truncated, full table size 1319 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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