|
| Status |
Public on Nov 03, 2012 |
| Title |
AC_E14 |
| Sample type |
RNA |
| |
|
| Source name |
4h culture of activated AC treated with E14
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Rosa25/OG2
cell type: Neural stem cell (NSC)-derived astrocytes (AC)
activated with: 1 µg/ml LPS and 10 ng/ml IFN-γ
treated with: hit compound E14 for 4hr
|
| Treatment protocol |
Medium was switched to DK10 and cells were activated with 1 µg/ml LPS and 10 ng/ml IFN-γ, followed by treatment with the indicated hit compound for 4 h. Cells were harvested directly on the culture dish.
|
| Growth protocol |
Astrocytes were differentiated from NSCs on gelatine in medium containing 10% FCS for 4 days and replated at a density of 1.5 x 1,000,000 cells/cm2 on Matrigel-coated cell culture dishes and matured for 14 days before treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion
|
| Label |
biotin
|
| Label protocol |
Ambion TotalPrep RNA Amplification Kit. RNA in put: 400ng, IVT: 12h
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions. Hybridization time: 18h
|
| Scan protocol |
Illumina iScan system using default settings
|
| Data processing |
Cubic Spline normalization with background subtraction in Illumina GenomeStudio V2010.3 with the module GeneExpression 1.8.0. Signal intensities below 10 were arbitrarily trimmed to the value of 10.
|
| |
|
| Submission date |
Apr 26, 2012 |
| Last update date |
Nov 03, 2012 |
| Contact name |
Susanne Höing |
| E-mail(s) |
s.hoeing@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE37611 |
Effect of small molecules on activated astrocytes |
| GSE37613 |
Effect of small molecules on activated astrocytes and microglia cells |
|
