|
| Status |
Public on Jul 22, 2013 |
| Title |
rat hippocampus (CA1 region), sham, 18 months old, subject 6 |
| Sample type |
RNA |
| |
|
| Source name |
rat hippocampus (CA1 region)_sham
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fisher 344
gender: male
age: 18 months
tissue: hippocampal CA1 brain
treament group: Sham (intact) control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from each CA1 tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water (Standard Affymetrix)
|
| Label |
biotin
|
| Label protocol |
For each sample, 20 ug of biotin-labeled cRNA was generated from 5 ug of total RNA according to manufacturer’s instructions (Standard Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix- 20 ug biotin-labeled cRNA was applied to a rat RGU34A GeneChip (Affymetrix) for hybridization (one chip per animal).
|
| Scan protocol |
Standard Affymetrix
|
| Data processing |
The data were analyzed for quality control with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Robust Multi-Chip Analysis (RMA) was used to generate signal intensity values using the standard settings in Bioconductor (2.2) operating in the R programming environment (R 2.7.1) with the 'justRMA' command.
|
| |
|
| Submission date |
Apr 26, 2012 |
| Last update date |
Jul 24, 2013 |
| Contact name |
Eric M Blalock |
| E-mail(s) |
eric.blalock@uky.edu
|
| Phone |
859-323-8033
|
| Organization name |
University of Kentucky
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Blalock
|
| Street address |
800 Rose St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40475 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE37618 |
Glucocorticoid-Dependent Hippocampal Transcriptome in Male Rats: Pathway-Specific Alterations with Aging |
|
