|
| Status |
Public on May 26, 2013 |
| Title |
bleo_week4_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse caudal lung lobe, bleomycin instillation, 4wk
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: female
tissue: caudal lung lobe
treatment: bleomycin instillation
time after treatment: 4 weeks
new collagen formation: 1.56%
|
| Treatment protocol |
Mice received intratracheal instillation of 30 μl bleomycin (1.25 U/ml) at timepoint 0. Mice received deuterated water (intraperitoneal injection of 35 μl deuterated water per gram body weight + replacement of drinking water with 8-10% deuterated water) during the 7 days before sacrifice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
The Illumina TotalPrep RNA Amplification Kit was used to synthesize biotin-labeled cRNA.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Data processing |
Background-subtracted data were submitted to quantile normalization in GeneSpring and log2 transformed.
|
| |
|
| Submission date |
Apr 27, 2012 |
| Last update date |
May 26, 2013 |
| Contact name |
Lars Verschuren |
| E-mail(s) |
lars.verschuren@tno.nl
|
| Phone |
+31888666039
|
| Organization name |
TNO
|
| Department |
Microbiology and Systems Biology
|
| Street address |
Utrechtseweg 48
|
| City |
Zeist |
| ZIP/Postal code |
3704HE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE37635 |
Collagen Dynamics and Transcriptional Profiling in Bleomycin-induced Pulmonary Fibrosis |
|
