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Sample GSM925045 Query DataSets for GSM925045
Status Public on Nov 25, 2013
Title patient2_fallopian_tube_rep2
Sample type RNA
 
Source name patient 2, fallopian tube epithelial cells, cultured, hTERT-immortalized
Organism Homo sapiens
Characteristics patient: 2
origin: fallopian tube epithelium
cell passage: 2
Growth protocol Cells were grown in newly developed WIT-fo medium (described in the associated publication)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNA Mini kits according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing The data were normalized using the vsnrma function in the package vsn.
 
Submission date Apr 29, 2012
Last update date Nov 25, 2013
Contact name Melissa Amy Merritt
Organization name Dana-Farber Cancer Institute
Department Biostatistics and Computational Biology
Lab Computational Biology and Functional Genomics (Quackenbush)
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215-5450
Country USA
 
Platform ID GPL570
Series (1)
GSE37648 Gene signatures of normal hTERT immortalized ovarian epithelium and fallopian tube epithelium (paired cultures from 2 donor patients)

Data table header descriptions
ID_REF
VALUE log2 vsnrma value

Data table
ID_REF VALUE
1007_s_at 10.79673723
1053_at 9.275010347
117_at 8.625163793
121_at 13.38290262
1255_g_at 7.202364098
1294_at 9.018182961
1316_at 7.843984238
1320_at 8.414474918
1405_i_at 7.300604838
1431_at 7.245843727
1438_at 8.686246889
1487_at 9.729127638
1494_f_at 8.806028723
1552256_a_at 9.214359863
1552257_a_at 11.43710178
1552258_at 7.402424764
1552261_at 7.83793722
1552263_at 8.379761704
1552264_a_at 10.12723716
1552266_at 7.316949958

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM925045_TI2010012733.CEL.gz 5.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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