|
| Status |
Public on Mar 07, 2006 |
| Title |
Src R388A/Y527F-12 hr |
| Sample type |
RNA |
| |
|
| Source name |
Src R388A/Y527F transfected SYF cells, 5 mM imidazole treated for 12 hr.
|
| Organism |
Mus musculus |
| Characteristics |
SYF (Src, Yes, Fyn deleted) mouse embryonic fibroblast cell line, stably transfected with mutant Src R388A/Y527F gene
|
| Biomaterial provider |
SYF cell line from ATCC
|
| Treatment protocol |
SrcR388A/Y527F stably transfected SYF cells were serum-starved overnight and then treated with imidazole 5 mM for 12 h.
|
| Growth protocol |
SYF cells stably transfected with Src constructs were cultured in DMEM media supplemented with 10% (v/v) FBS and 300 ug/ml of hygromycin B and incubated at 37°C in the presence of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Miniprep (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Affymetrix 1 round amplification protocol
|
| |
|
| Hybridization protocol |
18 hrs at 45 degree, with rotation 60 rpm
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 with GCOS 1.1 software
|
| Description |
Johns Hopkins JHMI Microarray Core
|
| Data processing |
Affymetrix GCOS 1.3 TGT Target Intensity Value150
|
| |
|
| Submission date |
Jan 13, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Philip A Cole |
| E-mail(s) |
yqiao1@jhmi.edu
|
| Phone |
410-614-0322
|
| Fax |
410-614-7717
|
| Organization name |
Johns Hopkins Medical School
|
| Department |
Pharmacology and Molecular Sciences
|
| Lab |
Dr. Philip A. Cole
|
| Street address |
725 N Wolfe St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4043 |
Gene profiling analysis of Src chemical rescue |
|
| Relations |
| Reanalyzed by |
GSE119085 |
