|
| Status |
Public on Oct 01, 2012 |
| Title |
Testes_Germ Cell-Specific Sin3a KO_rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
whole testes, Vasa-cre;Sin3a<deleted/floxed> mice (VSKO), biological replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
age: Postnatal Day 0
tissue: whole testis
strain: FVB.129/Sv mixed
genotype/variation: Ddx4(Vasa/Mvh)-cre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using the Rneasy Mini Kit (Qiagen) following the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
For the linear T7-based amplification step, 100 ng of each total RNA sample was used. To produce Cy3-labeled cRNA, the RNA samples were amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer's protocol. Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit. 600 ng Cy3-labeled fragmented cRNA in hybridization buffer was hybridized overnight (17 hours at 65 degrees C) to Agilent Whole Mouse Genome Oligo Microarrays 8x60K using Agilent's recommended hybridization chamber and oven.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1)
|
| Description |
Gene expression in total testicular tissue of a mouse lacking two functional Sin3a alleles in the germ cells at postnatal day 0
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization
|
| |
|
| Submission date |
May 21, 2012 |
| Last update date |
Oct 01, 2012 |
| Contact name |
Christopher J. Payne |
| E-mail(s) |
c-payne@northwestern.edu
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Street address |
2430 N. Halsted St.
|
| City |
Chicago |
| ZIP/Postal code |
60614 |
| Country |
USA |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE38096 |
Murine Testes: Germ Cell-Specific Sin3a Knockout vs. Hemizygous vs. Wild Type at Postnatal Day 0 |
|
