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Status |
Public on Oct 01, 2012 |
Title |
Testes_Germ Cell-Specific Sin3a Hemizygous_rep 2 |
Sample type |
RNA |
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Source name |
whole testes, Vasa-cre;Sin3a<deleted/+> mice (HEMI), biological replicate 2
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Organism |
Mus musculus |
Characteristics |
gender: male age: Postnatal Day 0 tissue: whole testis strain: FVB.129/Sv mixed genotype/variation: Sin3a hemizygous (HEMI)
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using the Rneasy Mini Kit (Qiagen) following the manufacturer's protocol.
|
Label |
Cy3
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Label protocol |
For the linear T7-based amplification step, 100 ng of each total RNA sample was used. To produce Cy3-labeled cRNA, the RNA samples were amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer's protocol. Yields of cRNA and the dye-incorporation rate were measured with the ND-1000 Spectrophotometer (NanoDrop Technologies).
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Hybridization protocol |
Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit. 600 ng Cy3-labeled fragmented cRNA in hybridization buffer was hybridized overnight (17 hours at 65 degrees C) to Agilent Whole Mouse Genome Oligo Microarrays 8x60K using Agilent's recommended hybridization chamber and oven.
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Scan protocol |
Scanned on an Agilent G2565AA scanner Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1)
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Description |
Gene expression in total testicular tissue of a mouse containing one functional Sin3a allele in the germ cells at postnatal day 0
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Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization
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Submission date |
May 21, 2012 |
Last update date |
Oct 01, 2012 |
Contact name |
Christopher J. Payne |
E-mail(s) |
c-payne@northwestern.edu
|
Organization name |
Northwestern University Feinberg School of Medicine
|
Street address |
2430 N. Halsted St.
|
City |
Chicago |
ZIP/Postal code |
60614 |
Country |
USA |
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Platform ID |
GPL13912 |
Series (1) |
GSE38096 |
Murine Testes: Germ Cell-Specific Sin3a Knockout vs. Hemizygous vs. Wild Type at Postnatal Day 0 |
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