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Status |
Public on May 23, 2012 |
Title |
Ms4a8a-transfected RAW264.7 co2 |
Sample type |
RNA |
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Source name |
Klon-8#1
|
Organism |
Mus musculus |
Characteristics |
cell type: Raw264.7 macrophages transfector: Ms4a8a cloneid: 8
|
Treatment protocol |
Ms4a8a-transfected and empty vector-transfected RAW264.7 cells were incubated for 48 h in DMEM medium supplemented with 10% FCS, 100 IU penicillin, 100 µg/ml streptomycin, 1% pyruvic acid, 1% non essential amino acids.
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Growth protocol |
Raw 264.7 cells were cultivated with DMEM medium supplemented with 10%FCS, 100IU penicillin, 100 µg/ml penicillin, 100 µg/ml streptomycin, 1% pyruvic acid, 1% non essential amino acids. Authentication of cell lines was assured by regular morphology checks and growth curve analyses. Cells were regularly tested for mycoplasma infection by PCR.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from these cultures using Qiagen RNeasy Kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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|
|
Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
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Scan protocol |
Affymetrix GeneArray Scanner3000
|
Description |
Gene expression from Ms4a8a transfected Raw264.7 macrophages
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Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
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Submission date |
May 23, 2012 |
Last update date |
May 23, 2012 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
|
Department |
ZMF
|
Street address |
Theodor-Kutzer-Ufer
|
City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
|
|
Platform ID |
GPL15041 |
Series (1) |
GSE38171 |
Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A. |
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