GEO help: Mouse over screen elements for information.
Status
Public on Jan 27, 2006
Title
M3C
Sample type
RNA
Source name
anterior eye
Organism
Mus musculus
Characteristics
Balb/c mouse Wild type, cornea
Extracted molecule
total RNA
Extraction protocol
limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type, cornea, limbus, epithelial basal cells
Data processing
Affymetrix Gene Operating System 1.2
Submission date
Jan 25, 2006
Last update date
Aug 28, 2018
Contact name
Mingyuan Zhou
E-mail(s)
m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name
Northwestern.edu
Department
Dermatology
Lab
9-031
Street address
303 E. Chicago Ave.
City
Chicago
State/province
IL
ZIP/Postal code
60611
Country
USA
Platform ID
GPL1261
Series (1)
GSE4098
Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by
GSE119085
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
15036.7
P
0.00141
AFFX-BioB-M_at
26662.7
P
0.000044
AFFX-BioB-3_at
25816.2
P
0.000044
AFFX-BioC-5_at
38052.8
P
0.000044
AFFX-BioC-3_at
18975.7
P
0.000044
AFFX-BioDn-5_at
33166.4
P
0.000044
AFFX-BioDn-3_at
106186
P
0.000044
AFFX-CreX-5_at
393265.4
P
0.000044
AFFX-CreX-3_at
344039.9
P
0.000044
AFFX-DapX-5_at
96.4
A
0.921998
AFFX-DapX-M_at
48.2
A
0.981719
AFFX-DapX-3_at
78.4
A
0.995002
AFFX-LysX-5_at
59.4
A
0.824672
AFFX-LysX-M_at
53.3
A
0.998898
AFFX-LysX-3_at
45.1
A
0.916408
AFFX-PheX-5_at
173.8
A
0.672921
AFFX-PheX-M_at
219.5
A
0.834139
AFFX-PheX-3_at
159.2
A
0.860518
AFFX-ThrX-5_at
132
A
0.960352
AFFX-ThrX-M_at
626.1
A
0.544587
Total number of rows: 45101 Table truncated, full table size 1227 Kbytes .Supplementary file
Size
Download
File type/resource
GSM93721.CEL.gz
5.6 Mb
(ftp) (http)
CEL
GSM93721.EXP.gz
365 b
(ftp) (http)
EXP