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Sample GSM93721 Query DataSets for GSM93721
Status Public on Jan 27, 2006
Title M3C
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, cornea
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 15036.7 P 0.00141
AFFX-BioB-M_at 26662.7 P 0.000044
AFFX-BioB-3_at 25816.2 P 0.000044
AFFX-BioC-5_at 38052.8 P 0.000044
AFFX-BioC-3_at 18975.7 P 0.000044
AFFX-BioDn-5_at 33166.4 P 0.000044
AFFX-BioDn-3_at 106186 P 0.000044
AFFX-CreX-5_at 393265.4 P 0.000044
AFFX-CreX-3_at 344039.9 P 0.000044
AFFX-DapX-5_at 96.4 A 0.921998
AFFX-DapX-M_at 48.2 A 0.981719
AFFX-DapX-3_at 78.4 A 0.995002
AFFX-LysX-5_at 59.4 A 0.824672
AFFX-LysX-M_at 53.3 A 0.998898
AFFX-LysX-3_at 45.1 A 0.916408
AFFX-PheX-5_at 173.8 A 0.672921
AFFX-PheX-M_at 219.5 A 0.834139
AFFX-PheX-3_at 159.2 A 0.860518
AFFX-ThrX-5_at 132 A 0.960352
AFFX-ThrX-M_at 626.1 A 0.544587

Total number of rows: 45101

Table truncated, full table size 1227 Kbytes.




Supplementary file Size Download File type/resource
GSM93721.CEL.gz 5.6 Mb (ftp)(http) CEL
GSM93721.EXP.gz 365 b (ftp)(http) EXP

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