GEO help: Mouse over screen elements for information.
Status
Public on Jan 27, 2006
Title
M5L
Sample type
RNA
Source name
anterior eye
Organism
Mus musculus Characteristics
Balb/c mouse
Extracted molecule
total RNA
Extraction protocol
limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type, cornea, limbus, epithelial basal cells
Data processing
Affymetrix Gene Operating System 1.2
Submission date
Jan 25, 2006
Last update date
Aug 28, 2018
Contact name
Mingyuan Zhou
E-mail(s)
m-zhou@northwestern.edu, r-lavker@northwestern.edu Organization name
Northwestern.edu
Department
Dermatology
Lab
9-031
Street address
303 E. Chicago Ave.
City
Chicago
State/province
IL
ZIP/Postal code
60611
Country
USA
Platform ID
GPL1261 Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
20499.3
P
0.001796
AFFX-BioB-M_at
38500.2
P
0.000044
AFFX-BioB-3_at
39363.4
P
0.000044
AFFX-BioC-5_at
59267.6
P
0.000044
AFFX-BioC-3_at
26561.9
P
0.000044
AFFX-BioDn-5_at
44953.9
P
0.000044
AFFX-BioDn-3_at
157736.9
P
0.000044
AFFX-CreX-5_at
607695.6
P
0.000044
AFFX-CreX-3_at
528005.8
P
0.000044
AFFX-DapX-5_at
310.9
A
0.824672
AFFX-DapX-M_at
39.5
A
0.953518
AFFX-DapX-3_at
39.2
A
0.993813
AFFX-LysX-5_at
40.2
A
0.979978
AFFX-LysX-M_at
383
A
0.574038
AFFX-LysX-3_at
120.5
A
0.824672
AFFX-PheX-5_at
73.7
A
0.987453
AFFX-PheX-M_at
96.4
A
0.957038
AFFX-PheX-3_at
1093.2
A
0.559354
AFFX-ThrX-5_at
72.9
A
0.973991
AFFX-ThrX-M_at
187.3
A
0.824672
45101 1228 Kbytes .Supplementary file Size Download File type/resource GSM93724.CEL.gz
5.4 Mb
(ftp) (http) CEL
GSM93724.EXP.gz
368 b
(ftp) (http) EXP
