GEO help: Mouse over screen elements for information.
Status
Public on Jan 27, 2006
Title
M5C
Sample type
RNA
Source name
anterior eye
Organism
Mus musculus Characteristics
Balb/c mouse
Extracted molecule
total RNA
Extraction protocol
limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type, cornea, limbus, epithelial basal cells
Data processing
Affymetrix Gene Operating System 1.2
Submission date
Jan 25, 2006
Last update date
Aug 28, 2018
Contact name
Mingyuan Zhou
E-mail(s)
m-zhou@northwestern.edu, r-lavker@northwestern.edu Organization name
Northwestern.edu
Department
Dermatology
Lab
9-031
Street address
303 E. Chicago Ave.
City
Chicago
State/province
IL
ZIP/Postal code
60611
Country
USA
Platform ID
GPL1261 Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
22336.1
P
0.002023
AFFX-BioB-M_at
38427.6
P
0.000081
AFFX-BioB-3_at
38811
P
0.000052
AFFX-BioC-5_at
58281.8
P
0.000044
AFFX-BioC-3_at
31545.4
P
0.000044
AFFX-BioDn-5_at
49561.8
P
0.000044
AFFX-BioDn-3_at
165693.7
P
0.000052
AFFX-CreX-5_at
645376.3
P
0.000052
AFFX-CreX-3_at
535865.6
P
0.000044
AFFX-DapX-5_at
73.5
A
0.989683
AFFX-DapX-M_at
154.6
A
0.737173
AFFX-DapX-3_at
196.1
A
0.860518
AFFX-LysX-5_at
215.5
A
0.824672
AFFX-LysX-M_at
232.3
A
0.699394
AFFX-LysX-3_at
1018.9
A
0.354453
AFFX-PheX-5_at
130.2
A
0.945787
AFFX-PheX-M_at
49.6
A
0.978098
AFFX-PheX-3_at
221.1
A
0.760956
AFFX-ThrX-5_at
486
A
0.772383
AFFX-ThrX-M_at
985.1
A
0.470241
45101 1229 Kbytes .Supplementary file Size Download File type/resource GSM93725.CEL.gz
5.4 Mb
(ftp) (http) CEL
GSM93725.EXP.gz
368 b
(ftp) (http) EXP
