GEO help: Mouse over screen elements for information.
Status
Public on Jan 27, 2006
Title
M6C
Sample type
RNA
Source name
anterior eye
Organism
Mus musculus Characteristics
Balb/c mouse
Extracted molecule
total RNA
Extraction protocol
limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type, cornea, limbus, epithelial basal cells
Data processing
Affymetrix Gene Operating System 1.2
Submission date
Jan 25, 2006
Last update date
Aug 28, 2018
Contact name
Mingyuan Zhou
E-mail(s)
m-zhou@northwestern.edu, r-lavker@northwestern.edu Organization name
Northwestern.edu
Department
Dermatology
Lab
9-031
Street address
303 E. Chicago Ave.
City
Chicago
State/province
IL
ZIP/Postal code
60611
Country
USA
Platform ID
GPL1261 Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
6127
P
0.000754
AFFX-BioB-M_at
10237.9
P
0.000052
AFFX-BioB-3_at
8542.5
P
0.000044
AFFX-BioC-5_at
14229.3
P
0.000044
AFFX-BioC-3_at
6591.3
P
0.000052
AFFX-BioDn-5_at
12178.9
P
0.000044
AFFX-BioDn-3_at
38508.1
P
0.000044
AFFX-CreX-5_at
145058.3
P
0.000044
AFFX-CreX-3_at
127822.1
P
0.000044
AFFX-DapX-5_at
18.6
A
0.937071
AFFX-DapX-M_at
6.7
A
0.99238
AFFX-DapX-3_at
121.3
A
0.631562
AFFX-LysX-5_at
216.5
A
0.368438
AFFX-LysX-M_at
14.4
A
0.997725
AFFX-LysX-3_at
72.8
A
0.672921
AFFX-PheX-5_at
11.4
A
0.995983
AFFX-PheX-M_at
11.2
A
0.945787
AFFX-PheX-3_at
251.8
A
0.51489
AFFX-ThrX-5_at
10.4
A
0.984817
AFFX-ThrX-M_at
41.2
A
0.834139
45101 1222 Kbytes .Supplementary file Size Download File type/resource GSM93726.CEL.gz
5.7 Mb
(ftp) (http) CEL
GSM93726.EXP.gz
363 b
(ftp) (http) EXP
