GEO help: Mouse over screen elements for information.
Status
Public on Jan 27, 2006
Title
M8L
Sample type
RNA
Source name
anterior eye
Organism
Mus musculus Characteristics
Balb/c mouse
Extracted molecule
total RNA
Extraction protocol
limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type, cornea, limbus, epithelial basal cells
Data processing
Affymetrix Gene Operating System 1.2
Submission date
Jan 25, 2006
Last update date
Aug 28, 2018
Contact name
Mingyuan Zhou
E-mail(s)
m-zhou@northwestern.edu, r-lavker@northwestern.edu Organization name
Northwestern.edu
Department
Dermatology
Lab
9-031
Street address
303 E. Chicago Ave.
City
Chicago
State/province
IL
ZIP/Postal code
60611
Country
USA
Platform ID
GPL1261 Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
962.9
P
0.000581
AFFX-BioB-M_at
1386.3
P
0.000044
AFFX-BioB-3_at
927.4
P
0.00006
AFFX-BioC-5_at
2173.1
P
0.00006
AFFX-BioC-3_at
1548.8
P
0.000044
AFFX-BioDn-5_at
2170.7
P
0.000044
AFFX-BioDn-3_at
6836.3
P
0.000052
AFFX-CreX-5_at
30473
P
0.000052
AFFX-CreX-3_at
27659
P
0.000044
AFFX-DapX-5_at
42.4
A
0.686292
AFFX-DapX-M_at
46.2
A
0.686277
AFFX-DapX-3_at
8.5
A
0.966335
AFFX-LysX-5_at
61.5
A
0.274972
AFFX-LysX-M_at
10.4
A
0.957038
AFFX-LysX-3_at
42.4
A
0.396911
AFFX-PheX-5_at
9.8
A
0.876428
AFFX-PheX-M_at
61.9
A
0.672921
AFFX-PheX-3_at
56
A
0.455413
AFFX-ThrX-5_at
10.1
A
0.989683
AFFX-ThrX-M_at
10
A
0.617401
45101 1221 Kbytes .Supplementary file Size Download File type/resource GSM93731.CEL.gz
5.4 Mb
(ftp) (http) CEL
GSM93731.EXP.gz
368 b
(ftp) (http) EXP
