|
| Status |
Public on Jan 21, 2014 |
| Title |
P4KO rescued with PMCA4b late G1/S replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
carotid artery; male mouse
|
| Organism |
Mus musculus |
| Characteristics |
transfection: PMCA4b
cell type: primary vascular smooth muscle cells
genotype: PMC4 KO
genetic background: C57bl6
gender: male
cell cycle stage: Late G1/S stage
|
| Treatment protocol |
Cells were stimulated with serum and PDGF-BB and harvested at the late G1/S stage of the cell cycle.
|
| Growth protocol |
P4WT and P4KO primary VSMC were serum synchronized. Alternatively, P4KO cells electroporated with PMCA4b or Vector DNA were serum synchronized.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Life Technologies PicoPure RNA extraction kit and quantified by Nanodrop.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amplification Kit Cat.# IL 1791 for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
PMCA4 knockout C57bl6 male mouse
replicate 5
|
| Data processing |
The data were normalised using quantile normalisation with lumi in R
|
| |
|
| Submission date |
May 30, 2012 |
| Last update date |
Jan 21, 2014 |
| Contact name |
Mansoor Husain |
| E-mail(s) |
mansoor.husain@uhn.ca
|
| Phone |
416-581-7489
|
| Organization name |
University Health Network
|
| Department |
Toronto Gen. Hosp. Res Institute
|
| Lab |
Mansoor Husain lab
|
| Street address |
Room TMDT-3-910, 101 College St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE38319 |
Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 wild type (P4WT), PMCA4 knockout(P4KO), P4KO rescued with PMCA4b and P4KO rescued with Vector primary vascular smooth muscle cells |
| GSE38320 |
Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells |
|
