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Sample GSM938951 Query DataSets for GSM938951
Status Public on Jan 21, 2014
Title P4KO rescued with Vector B late G1/S replicate 2
Sample type RNA
 
Source name carotid artery; male mouse
Organism Mus musculus
Characteristics transfection: vector control
cell type: primary vascular smooth muscle cells
genotype: PMC4 KO
genetic background: C57bl6
gender: male
cell cycle stage: Late G1/S stage
Treatment protocol Cells were stimulated with serum and PDGF-BB and harvested at the late G1/S stage of the cell cycle.
Growth protocol P4WT and P4KO primary VSMC were serum synchronized. Alternatively, P4KO cells electroporated with PMCA4b or Vector DNA were serum synchronized.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Life Technologies PicoPure RNA extraction kit and quantified by Nanodrop.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amplification Kit Cat.# IL 1791 for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description PMCA4 knockout C57bl6 male mouse
replicate 2
Data processing The data were normalised using quantile normalisation with lumi in R
 
Submission date May 30, 2012
Last update date Jan 21, 2014
Contact name Mansoor Husain
E-mail(s) mansoor.husain@uhn.ca
Phone 416-581-7489
Organization name University Health Network
Department Toronto Gen. Hosp. Res Institute
Lab Mansoor Husain lab
Street address Room TMDT-3-910, 101 College St.
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL6885
Series (2)
GSE38319 Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 wild type (P4WT), PMCA4 knockout(P4KO), P4KO rescued with PMCA4b and P4KO rescued with Vector primary vascular smooth muscle cells
GSE38320 Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval-VecB-#2

Data table
ID_REF VALUE Detection Pval-VecB-#2
ILMN_2896528 11.78724374 0
ILMN_2721178 9.536642508 0
ILMN_3033922 10.2256391 0
ILMN_3092673 12.23343496 0
ILMN_2816356 7.108543475 0.002506266
ILMN_2808939 11.26121253 0
ILMN_2634564 9.729783567 0
ILMN_2737647 5.611632319 0.4385965
ILMN_2734484 9.01015107 0
ILMN_2952292 7.603169385 0
ILMN_2699078 4.852943667 0.9010025
ILMN_1213681 8.873198388 0
ILMN_2735413 5.389769953 0.5914787
ILMN_2735415 5.947807805 0.2180451
ILMN_2891688 9.981844882 0
ILMN_2637698 11.04659545 0
ILMN_2674228 8.541190694 0
ILMN_2601546 6.450994219 0.04511278
ILMN_1230831 5.504609661 0.5112782
ILMN_2848071 6.182718615 0.1065163

Total number of rows: 25697

Table truncated, full table size 809 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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