|
| Status |
Public on Jan 14, 2013 |
| Title |
Hardy Fraction B cell, rep 5 |
| Sample type |
RNA |
| |
|
| Source name |
Hardy Fraction B cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Pax5 wildtype
cell type: flow sorted B cell precursor populations
fraction: Hardy Fraction B
|
| Treatment protocol |
None
|
| Growth protocol |
RNA was extracted from flow sorted B cell subpopulations from Pax5 wild type C57BL/6 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Arcturus PicoPure
|
| Label |
biotin
|
| Label protocol |
RNA amplification and labeling was performed using the Nugen Ovation Pico Sustem
|
| |
|
| Hybridization protocol |
Following fragmentation, 8 microgram of cRNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Pax5WT_FractionB5
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jun 04, 2012 |
| Last update date |
Jan 14, 2013 |
| Contact name |
Shann-Ching Chen |
| E-mail(s) |
Shann-Ching.Chen@stjude.org
|
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Pathology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE38463 |
Gene expression profiling of mouse B cell progenitors |
|
