|
| Status |
Public on May 28, 2015 |
| Title |
MEP_C2 |
| Sample type |
RNA |
| |
|
| Source name |
Freshly isolated mammary epithelial cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Freshly isolated mammary epithelial cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by RNA STAT-60TM solution according to manufacturer's instruction (Tel-Test, INC).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
07-28-11_C_Deng_YL#2_Mo_Gene1_0_ST_MEP_C2
|
| Data processing |
RMA by software Partek
|
| |
|
| Submission date |
Jun 04, 2012 |
| Last update date |
May 28, 2015 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE38471 |
Enhancing Mammary Differentiation by Overcoming Lineage Specific Epigenetic Modification and Signature Gene Expression of Fibroblast Derived iPSCs |
|
