|
Status |
Public on May 28, 2015 |
Title |
IPS-MEP_1 |
Sample type |
RNA |
|
|
Source name |
differentiated inducible pluripotent stem cells derived from mammary epithelial cells
|
Organism |
Mus musculus |
Characteristics |
cell type: differentiated inducible pluripotent stem cells derived from mammary epithelial cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by RNA STAT-60TM solution according to manufacturer's instruction (Tel-Test, INC).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
07-28-11_C_Deng_YL#4_Mo_Gene1_0_ST_IPS-MEP_1
|
Data processing |
RMA by software Partek
|
|
|
Submission date |
Jun 04, 2012 |
Last update date |
May 28, 2015 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL10740 |
Series (1) |
GSE38471 |
Enhancing Mammary Differentiation by Overcoming Lineage Specific Epigenetic Modification and Signature Gene Expression of Fibroblast Derived iPSCs |
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