|
| Status |
Public on Dec 31, 2012 |
| Title |
Olig2 Cells, PDGF Glioma Total RNA, Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Olig2 Cells, PDGF Glioma Total RNA, Replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
fraction: Total RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For collection of total cellular RNA, normal brain or grossly-dissected tumor tissue was dissociated into a single cell suspension with papain and GFP+ cells were collected by FACS (Becton-Dickinson Aria Cell Sorter). Sorted cells were collected with Trizol-LS (Invitrogen), chloroform extracted, precipitated with sodium acetate in isopropanol overnight, and purified with Qiagen RNeasy MinElute Cleanup Kit. Ribosome-bound RNA was immunoprecipitated with anti-GFP conjugated magnetic beads from mechanically-homogenized tissue and purified as above. Tissue samples were divided equally for total and ribosome-bound RNA to generate paired datasets.
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification manufacturer protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
PDGF Total G3
|
| Data processing |
Non-normalized data is background subtracted; Normalized data is background subtracted, filtered for only probes with detection p-value<0.05 in all samples, quantile normalized in Partek Genomics software and log2 transformed.
|
| |
|
| Submission date |
Jun 08, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Karim Helmy |
| E-mail(s) |
karimhelmy@stanfordalumni.org
|
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE38591 |
Identification of global alteration of translational regulation in glioma in vivo |
|
