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Status |
Public on Jun 13, 2013 |
Title |
MN1 full construct |
Sample type |
RNA |
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Source name |
Common Myeloid Progenitor (CMP) cells transduced with full-length MN1 expression vector
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Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6
|
Treatment protocol |
Linage negative mouse bone marrow cells were cultured for a total of 5 days (including the transduction period) in growth medium which contains IMDM+glutamine (2mM)+penicillin/streptomycin (each 100 U/mL)+10% heat inactivated fetal bovine serum+50ng/ml mouse SCF+ 10ng/ml mouse IL3+ 10ng/ml mouse IL6.
|
Growth protocol |
MACS-sorted lineage negative mouse bone marrow cells were transduced with retroviral vectors that expressed full-length or truncated forms of MN1 protein. Cells were cultured total 5 days (including the transduction period), and CMP+/GFP+ cells were FACS-sorted 2 days after the first transduction.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix eukaryote two-cycle expression protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004-2009, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 6 ug of cRNA were hybridized for 16 hr at 45C on a HT MG-430 PM Genome Array. GeneChip plate arrays were washed and stained in the Affymetrix GeneTitan system.
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Scan protocol |
Gene array plates were scanned using the Affymetrix GeneTitan system.
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Data processing |
The data were analyzed with the Affymetrix Expression Console software v1.1. RMA normalization and summarization was performed using the default settings in the software.
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Submission date |
Jun 17, 2012 |
Last update date |
Jun 13, 2013 |
Contact name |
Geoffrey Neale |
E-mail(s) |
geoffrey.neale@stjude.org
|
Organization name |
St Jude Childrens Research Hospital
|
Department |
Hartwell Center
|
Street address |
262 Danny Thomas Place
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE38767 |
Gene expression profiles in mouse common myeloid progenitor (CMP) and leukemia cells expressing full-length MN1 or truncated versions of MN1 protein |
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