|
| Status |
Public on Jun 29, 2012 |
| Title |
siRae1 rwt infected replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
siRae1 rwt infected replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
|
| Treatment protocol |
Cells were transfected with Rae1 siRNA or NT siRNA using Hiperfect transfection reagent (Qiagen Corporation). At 72 hours post transfection with Rae1 siRNA or NT siRNA, cells were mock-infected or infected with rwt virus at MOI= 30. At 6 h postinfection total RNA was extracted
|
| Growth protocol |
HeLa cells were cultured in Dulbecco's modified Eagle's medium containing 7% fetal bovine serum and 2 mM glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
RNA was labeled with the 3'-IVT Labeling Kit (Affymetrix) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
RNA hybridization was conducted with GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays (Affymetrix, USA) using the GeneAtlas System.
|
| Scan protocol |
Arrays were scanned by the GeneAtlas Imaging Station incorporated on the GeneAtlas System.
|
| Data processing |
Data was generated by the GeneAtlas Instrument Control Software. The raw intensity data were normalized using Systematic Variation Normalization.
|
| |
|
| Submission date |
Jun 21, 2012 |
| Last update date |
Jun 29, 2012 |
| Contact name |
Jeff Chou |
| E-mail(s) |
jchou@wakehealth.edu
|
| Phone |
3367572617
|
| Organization name |
Atrium Health Wake Forest Baptist
|
| Street address |
Medical Center Blvd
|
| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE38866 |
Expression Data from siRae1 and siNT infected with VSV or mock infected |
|
