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Sample GSM951041 Query DataSets for GSM951041
Status Public on Jun 29, 2012
Title siRae1 mock infected replicate 2
Sample type RNA
 
Source name siRae1 mock infected replicate 2
Organism Homo sapiens
Characteristics cell line: HeLa
Treatment protocol Cells were transfected with Rae1 siRNA or NT siRNA using Hiperfect transfection reagent (Qiagen Corporation). At 72 hours post transfection with Rae1 siRNA or NT siRNA, cells were mock-infected or infected with rwt virus at MOI= 30. At 6 h postinfection total RNA was extracted
Growth protocol HeLa cells were cultured in Dulbecco's modified Eagle's medium containing 7% fetal bovine serum and 2 mM glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol RNA was labeled with the 3'-IVT Labeling Kit (Affymetrix) according to manufacturer's instructions.
 
Hybridization protocol RNA hybridization was conducted with GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays (Affymetrix, USA) using the GeneAtlas System.
Scan protocol Arrays were scanned by the GeneAtlas Imaging Station incorporated on the GeneAtlas System.
Data processing Data was generated by the GeneAtlas Instrument Control Software. The raw intensity data were normalized using Systematic Variation Normalization.
 
Submission date Jun 21, 2012
Last update date Jun 29, 2012
Contact name Jeff Chou
E-mail(s) jchou@wakehealth.edu
Phone 3367572617
Organization name Atrium Health Wake Forest Baptist
Street address Medical Center Blvd
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
 
Platform ID GPL13667
Series (1)
GSE38866 Expression Data from siRae1 and siNT infected with VSV or mock infected

Data table header descriptions
ID_REF
VALUE normalized log2 converted intensity

Data table
ID_REF VALUE
11715100_at 5.9306884
11715101_s_at 6.4830527
11715102_x_at 6.0449276
11715103_x_at 5.3880134
11715104_s_at 5.55274
11715105_at 4.5985723
11715106_x_at 5.1396074
11715107_s_at 5.105134
11715108_x_at 5.3176312
11715109_at 5.5028257
11715110_at 5.4036307
11715111_s_at 6.815513
11715112_at 4.4980493
11715113_x_at 6.6666503
11715114_x_at 6.654375
11715115_s_at 3.673134
11715116_s_at 4.9572954
11715117_x_at 3.6113303
11715118_s_at 5.3457513
11715119_s_at 4.7927146

Total number of rows: 49386

Table truncated, full table size 1108 Kbytes.




Supplementary file Size Download File type/resource
GSM951041_Si_RaeI_mock_6_30_11.ga.cel.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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