|
| Status |
Public on Jul 01, 2012 |
| Title |
AND.Rag1-/-H-2d DP thymocytes_gp250/I-Ek stimulation_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Pre-selected DP thymocytes from AND.Rag1-/-H-2d mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Pre-selected DP thymocyte
genotype/variation: AND.Rag1-/-H-2d
agent: gp250/I-Ek
protocol: positive selection signals
|
| Treatment protocol |
1x106 thymocytes were stimulated with plate bound peptide loaded I-Ek Ig dimers for seven hours
|
| Growth protocol |
Pre-selected thymocytes were cultured in Iscove's DMEM containing 10% FCS, 1x nonessential amino acid, 2 mM GlutaMax, 1 mM sodium pyruvate, 0.05% gentamicin, 50 µM 2-mercaptoethanol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kits (Qiagene) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Gene expression data from pre-selected DP cells from gp250/I-Ek stimulation (positive selection signals)
SAMPLE 2
|
| Data processing |
Data was normalized and modeled using dChip software.
|
| |
|
| Submission date |
Jun 25, 2012 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Wan-Lin Lo |
| Organization name |
Washington University in St Louis
|
| Street address |
425 S Euclid Ave, campus box 8118
|
| City |
Saint Louis |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE38909 |
Transcriptional profiling analysis of pre-selected DP thymocytes in response to positive and negative selection signals |
|
