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Sample GSM953138 Query DataSets for GSM953138
Status Public on Jun 27, 2012
Title COPD [220968_miRNA]
Sample type RNA
 
Source name Lung tissue from smoker with COPD
Organism Homo sapiens
Characteristics diagnosis: COPD
fvc-group: 2
gold stage: 2
age: 67
gender (1=male, 2=female): 2
pkyrs: 10
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen tissue samples using a freeze fracture protocol, followed by Trizol extraction and precipitation at -20ºC overnight to increase the yield of small RNAs. RNA integrity was determined by capillary electrophoresis on an Agilent 2100 Bioanalyzer. Only RNA with an integrity number $7 was used in the profiling studies.
Label Cy3
Label protocol miRNAs were labeled according to the manufacturer's instruction (Exiqon, Woburn, Massachusetts, USA)
 
Hybridization protocol The labelled miRNA probes were hybridized to the miRCURY LNA arrays v11.0 for 16h at 56ºC
Scan protocol Hybridized slides were washed and scanned with ScanArray Express (Perkin Elmer)
Description COPD
Data processing miRNA expression data was consolidated by taking median value across replicated probes. Then, it was normalized by quantile method, and transformed in log2-scale
 
Submission date Jun 27, 2012
Last update date Jun 27, 2012
Contact name Ji-Hoon Cho
E-mail(s) jcho@systemsbiology.org
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL7723
Series (1)
GSE38974 Gene expression networks in COPD: microRNA and mRNA regulation

Data table header descriptions
ID_REF
VALUE Log2-normalized (quantile normalization) signal intensity

Data table
ID_REF VALUE
1100 6.4026281
3320 12.723235
3980 6.5282532
4040 6.1506199
4390 6.3827488
4610 12.816844
4700 7.1859953
5250 7.5418046
5560 9.8590031
5730 6.2456261
5740 10.527465
6880 6.3001565
9578 9.6663774
9938 9.9140722
10138 10.546531
10306 9.2661072
10482 6.34439
10618 7.0007817
10901 6.4491335
10902 6.1357281

Total number of rows: 1981

Table truncated, full table size 30 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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