|
| Status |
Public on Sep 01, 2012 |
| Title |
WT1 |
| Sample type |
SRA |
| |
|
| Source name |
third instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: wildtype - Oregon R
developmental stage: third instar larvae
|
| Growth protocol |
Fly stocks were maintained on standard cornmeal media at 25ºC with 70% humidity. Mutant 3rd instar larvae lacking HP1a were recovered from a cross of flies carrying the Su(var)205^04 allele over a GFP balancer to flies carrying the Su(var)205^05 allele over a GFP balancer by selecting for lack of GFP.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from third instar larvae using Trizol according to the manufacturer's recommendations. rRNA-depleted cDNA libraries suitable for Illumina sequencing were prepared and sequencing was carried out by the Genome Technology Access Center (GTAC) at Washington University according to standard protocols. Following are the index sequences used and the samples they correspond to. CCATCAT HP1a -/- 1; CCGATTA OR 2; CTTGGAA OR 1; TAACAAG HP1a -/- 2.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Alignment: Tophat
Gene level expression: Cufflinks
Genome_build: R5.32
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
|
| |
|
| Submission date |
Jul 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicole Christina Riddle |
| Organization name |
Washington University
|
| Department |
Biology
|
| Lab |
SCR Elgin
|
| Street address |
1 Brookings Dr.; CB1137
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63130 |
| Country |
USA |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE39083 |
RNA expression in Drosophila melanogaster mutants lacking HP1a |
|
| Relations |
| Reanalyzed by |
GSM3278689 |
| SRA |
SRX157651 |
| BioSample |
SAMN01085583 |
