|
| Status |
Public on Feb 09, 2006 |
| Title |
IL-2 KO + IL-2 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ GFP-Foxp3+ from IL-2-null
|
| Organism |
Mus musculus |
| Characteristics |
Sample isolated from CD4+GFPFoxp3+ T cells isolated from IL2-null mice after treatment with recombinant IL-2
|
| Treatment protocol |
24 d old Il2-/- mice were injected intraperitoneally with 50 ng/g body weight of recombinant human IL-2 in PBS every 8 h for 24 h (total of four injections). After 24 h, lymph node and spleen suspensions were subjected to a combination of MACS and FACS sorting for RNA isolation as described in Nat Immunol. 2005 Nov;6(11):1142-51.
|
| Extracted molecule |
total RNA |
| Label |
Biotin
|
| |
|
| Description |
Sample isolated from CD4+GFPFoxp3+ T cells isolated from IL2-null mice after treatment with recombinant IL-2.
24 d old Il2-/- mice were injected intraperitoneally with 50 ng/g body weight of recombinant human IL-2 in PBS every 8 h for 24 h (total of four injections). After 24 h, lymph node and spleen suspensions were subjected to a combination of MACS and FACS sorting for RNA isolation as described in Nat Immunol. 2005 Nov;6(11):1142-51.
|
| Data processing |
GCRMA v 1.1.3, default settings
|
| |
|
| Submission date |
Feb 03, 2006 |
| Last update date |
Feb 08, 2006 |
| Contact name |
Jeffrey Rasmussen |
| E-mail(s) |
rasmuss@u.washington.edu
|
| Phone |
2065439058
|
| Organization name |
University of Washington
|
| Department |
Immunology
|
| Lab |
Rudensky
|
| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4179 |
A function for interleukin 2 in Foxp3-expressing regulatory T cells |
|
