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Sample GSM95621 Query DataSets for GSM95621
Status Public on Feb 17, 2006
Title Zebrafish_Wild Type_miR-430 injected_Rep2
Sample type RNA
 
Source name Wild Type embryos_miR-430 injected
Organism Danio rerio
Characteristics TLAB (wild type) embryos, 9 hours post fertilization

injected with miR-430 microRNA at the one cell stage
Biomaterial provider Antonio Giraldez/Alex Schier
Treatment protocol injected with miR-430 microRNA at the one cell stage
Growth protocol embryos were collected and grown in egg water (Stock salts: 40 g "Instant Ocean" Sea Salts added to 1 L distilled water
Egg water = 1.5 ml stock salts added to 1 L distilled water = 60 µg/ml final concentration) at 28 degrees Celcius prior to RNA extraction
Extracted molecule total RNA
Extraction protocol RNA was isolated from 20 embyros using Trizol (Invitrogen). 20-50 ng of sample was quality checked using the RNA 6000 NanoAssay and Bioanalyzer 2100 (Agilent). Samples with a 28S/18S ribosomal peak ratio of 1.8-2.0 were labled.
Label biotin
Label protocol 2 micrograms of total RNA was used for cDNA synthesis using an oligo(dT)-T7 primer and the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen). Synthesis, linear amplification, and labeling of cRNA were accomplished by in vitro transcription using the MessageAmp aRNA Kit (Ambion) and biotinylated nucleotides(Enzo Diagnostics).
 
Hybridization protocol 10 micrograms of labeled and fragmented cRNA were hybridized to GeneChip at 45 degrees C for 16 hours. Post hybridization staining and washing were performed according to the manufacturer (Affymetrix).
Scan protocol GeneChips were scanned with a high-numerical Aperture and flying objective (FOL) lens in a GS3000 scanner (Affymetrix).
Description --
Data processing normalization of all arrays was performed by dChip v.3.1. The values were then calculated using dChip's PM/MM difference model (www.dchip.org)
 
Submission date Feb 05, 2006
Last update date Jun 03, 2013
Contact name Antonio J. Giraldez
E-mail(s) antonio.giraldez@yale.edu
Phone 203 785 5423
Organization name Yale University
Department Genetics
Lab Giraldez Lab
Street address 333 Cedar Street
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
 
Platform ID GPL1319
Series (1)
GSE4201 Zebrafish microRNA miR-430 promotes deadenylation and clearance of maternal mRNAs

Data table header descriptions
ID_REF
VALUE normalized across all samples by dChip (v1.3) and calculated using the dChip PM/MM Difference Model
ABS_CALL Absolute Call (P= present, A= Absent, M= marginal)
STANDARD ERROR Standard error of VALUE determined by dChip (v1.3)

Data table
ID_REF VALUE ABS_CALL STANDARD ERROR
AFFX-BioB-5_at 260.824 P 9.58972
AFFX-BioB-M_at 232.419 P 7.05658
AFFX-BioB-3_at 254.559 P 4.73394
AFFX-BioC-5_at 662.264 P 9.30527
AFFX-BioC-3_at 620.161 P 15.1577
AFFX-BioDn-5_at 1278.73 P 24.3508
AFFX-BioDn-3_at 3058.3 P 68.633
AFFX-CreX-5_at 8088.89 P 194.062
AFFX-CreX-3_at 9488 P 295.556
AFFX-DapX-5_at 106.446 A 3.54013
AFFX-DapX-M_at 58.8372 A 3.50706
AFFX-DapX-3_at 44.7697 A 4.18829
AFFX-LysX-5_at 54.5176 A 2.18651
AFFX-LysX-M_at 76.1791 A 2.49534
AFFX-LysX-3_at 72.9406 A 9.50831
AFFX-PheX-5_at 262.752 A 4.59289
AFFX-PheX-M_at 476.301 A 3.80594
AFFX-PheX-3_at 268.213 A 9.16949
AFFX-ThrX-5_at 80.1378 A 2.5588
AFFX-ThrX-M_at 173.518 A 3.02202

Total number of rows: 15617

Table truncated, full table size 526 Kbytes.




Supplementary file Size Download File type/resource
GSM95621.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM95621.EXP.gz 345 b (ftp)(http) EXP
Raw data provided as supplementary file

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