|
| Status |
Public on Jan 29, 2013 |
| Title |
PML Knockout Mice fed Low Fat Diet 3 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
genotype: PML Knockout
treatment: Low Fat Diet
gender: male
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Trizol Protocol.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard methods were applied using the high throughput protocal for PM arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained using Standard protocol.
|
| Scan protocol |
Affymetrix High-throughtput scanner standard protocol
|
| Description |
PML Knockout Mice fed Low Fat Diet
|
| Data processing |
Data was normalized by a robust multi-chip analysis (RMA).
|
| |
|
| Submission date |
Jul 10, 2012 |
| Last update date |
Jan 29, 2013 |
| Contact name |
Manoj Bhasin |
| Phone |
6176670009
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Street address |
330 Brookline Avenue RN 380E
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE39220 |
PML regulates PPAR signaling and fatty acid oxidation in breast cancer |
|
