|
Status |
Public on Jan 29, 2013 |
Title |
Wild Type Mice fed high fat diet 2 |
Sample type |
RNA |
|
|
Source name |
Liver
|
Organism |
Mus musculus |
Characteristics |
genotype: Wild Type treatment: high fat diet gender: male tissue: liver
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted using Trizol Protocol.
|
Label |
biotin
|
Label protocol |
Affymetrix standard methods were applied using the high throughput protocal for PM arrays
|
|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained using Standard protocol.
|
Scan protocol |
Affymetrix High-throughtput scanner standard protocol
|
Description |
Wild Type Mice fed high fat diet
|
Data processing |
Data was normalized by a robust multi-chip analysis (RMA).
|
|
|
Submission date |
Jul 10, 2012 |
Last update date |
Jan 29, 2013 |
Contact name |
Manoj Bhasin |
Phone |
6176670009
|
Organization name |
Beth Israel Deaconess Medical Center
|
Street address |
330 Brookline Avenue RN 380E
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE39220 |
PML regulates PPAR signaling and fatty acid oxidation in breast cancer |
|