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Sample GSM960720 Query DataSets for GSM960720
Status Public on Oct 18, 2012
Title WT control for Tg eWAT biological replicate 3
Sample type RNA
 
Source name epididymal white adipose tissue
Organism Mus musculus
Characteristics genotype: Wild-type
genetic background: C57Bl/6J
age: 3 months
treatment: fed ad libitum
tissue: epididymal white adipose tissue
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description biological replicate 3
7917911014_G
Data processing Image data were converted into unnormalized Sample Probe Profiles using the Illumina BeadStudio software and processed using the Model-Based Background Correction method (Xie, Wang, & Story, 2009). All gene expression values were log2 transformed and quantile normalized. Significance Analysis of Microarrays (Tusher, Tibshirani, & Chu, 2001) was used to identify differentially expressed genes among different groups using a false discovery rate of less than 10% and fold fold-change >2 as criteria.
 
Submission date Jul 12, 2012
Last update date Oct 18, 2012
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL6885
Series (1)
GSE39313 The role of FGF21 in aging and aging-related diseases

Data table header descriptions
ID_REF
VALUE log2 quantile normalized

Data table
ID_REF VALUE
ILMN_1250052 9.39
ILMN_3122480 9.3
ILMN_2599935 9.47
ILMN_2675543 9.33
ILMN_2686883 9.33
ILMN_2751818 9.32
ILMN_2728634 9.34
ILMN_3040515 9.33
ILMN_2711608 9.32
ILMN_1232875 9.36
ILMN_1258507 9.33
ILMN_2746142 9.3
ILMN_1252690 9.35
ILMN_2655499 9.31
ILMN_1252870 9.33
ILMN_1248179 9.32
ILMN_2649955 9.37
ILMN_2628708 9.31
ILMN_3024781 9.32
ILMN_2705628 9.36

Total number of rows: 25697

Table truncated, full table size 452 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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